Other

Part:BBa_I716025:Design

Designed by: Arthur Yu   Group: iGEM07_Berkeley_UC   (2007-08-03)


VHb (NcoI-EcoRI in pETDUET)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

THIS IS NOT BIOBRICK 

making I716025 - VHb vitreoscilla hemoglobin basic part
NdeI/BglII petDUET1 style

[pcr] AY017/18 onto pRED2   (460 bp, NdeI/BglII)
put it into petDUET1
you get I716025

AY017 // catggCATatgttagaccagcaaacc
AY018 // gtacaAGATCTttattcaaccgcttgagcg


Source

pRED2

References